Source:
A New Zealand rabbit was immunized sub-cutaneously with a KLH coupled peptide (Neosystems, France).
Applications:
This antibody can be used for immunohistochemistry and western blotting.
Specificity:
1. Western-blot : in homogenates from brain of AD patients, this antibody mostly recognizes normal and pathological tau proteins that are phosphorylated on serine 199.
2. Immunohistochemistry : this antibody recognizes the early changes of neurofibrillary degeneration (tangles and dystrophic neurites, neuritic plaques) on frozen sections of Alzheimer's disease cortical brain tissue. Excellent also on paraffin sections.
Excellent for transgenic mice that develop a tau pathology
Presentation:
This rabbit polyclonal antibody to human Tau proteins is in 50% glycerol. There is no other additive or preservative. It is a total serum.
Instructions for use:
1. For Western-blot, a final dilution of 1/10000 in TBS-Tween buffer + 5% non-fat dry milk is useful to detect Tau proteins in cortical brain homogenates.
2. For immunohistochemistry, a final dilution of 1/1000 (in Coons buffer) is suggested for immunostaining on frozen sections of cortical brain tissue.
Note: The recommended concentrations are approximative values. For each application, a dose response assay should be performed to determine the optimal concentration for use.
FOR RESEARCH USE ONLY
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References :
[1] Goedert M. and Jakes R., EMBO J. (1990); 9, 4225-4230
See also the results on tau transgenic mice (Gotz et
al).