This polyclonal antibody is raised against a synthetic peptide corresponding to the last 15 amino-acids of tau protein.
Source:
A New Zealand rabbit was immunized sub-cutaneously with a KLH coupled peptide (Neosystems, France).
Applications:
This antibody can be used for immunohistochemistry and western blotting.
Specificity:
1. Western-blot : in homogenates from brain of AD patients, this antibody recognizes native as well as pathological tau proteins.
2. Immunohistochemistry : this antibody recognizes neurofibrillary tangles and dystrophic neurites on frozen sections of Alzheimer's disease cortical brain tissue. Excellent also on paraffin sections.
Presentation:
This rabbit polyclonal antibody to human Tau proteins is in 50% glycerol. There is no other additive or preservative. It is a total serum.
Instructions for use:
1. For Western-blot, a final dilution of 1/2.000 in TBS-Tween buffer + 5% non-fat dry milk is useful to detect Tau proteins in cortical brain homogenates.
2. For immunohistochemistry, a final dilution of 1/2000 (in Coons buffer) is suggested for immunostaining on frozen sections of cortical brain tissue.
Note: The recommended concentrations are approximative values. For each application, a dose response assay should be performed to determine the optimal concentration for use.
FOR RESEARCH USE ONLY
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References :
- Goedert M. and Jakes R., EMBO J. (1990); 9, 4225-4230
Many of our papers including:
| - Sergeant N, Sablonniere B, Schraen-Maschke S, Ghestem A, Maurage CA, Wattez A, Vermersch P, Delacourte A. | Related Articles |
