We have an excellent monoclonal antibody that only detects gamma NSE in total human brain homogenates, using 1D and 2D- western blots .

Useful to probe NSE as an internal marker of neuronal proteins, allowing precise quantification.

Useful to quantify neuronal loss in the CSF (cerebrospinal fluid), for neurodegenerative disorders, including Creutzfeld Jacob disease, bovine spongiform encephalopathy.

This antibody is at your disposal, if there is a feed back of information
To be used at a dilution of 1/2000 to 1/10.000

Anti-gamma -enolase = Anti Neuron Specific Enolase

Enolases are cytoplasmic glycolytic enzymes. Three isoenzymes a-, ß- and g -enolase have been identified. The g -enolase, also called neuron-specific enolase (NSE), is expressed only in neurons and neuron-derived cells.

Mouse monoclonal antibody 1C1 is specific for NSE.

Presentation

The antibody is conserved as ascite fluid in 50% vol/vol glycerol. It does not contain other conservative adducts. It can be stored at -20°C for several months

Applications

This antibody can be used for Western-Blotting, immunocytochemistry or immunofluorescence.

Specificity

On Western-Blots of human brain tissue homogenates and neuronal cell cultures, 1C1 antibody detects a single band at 47 kDa (figure 1).

On two-dimensional gel electrophoresis followed by Western-Blot, a major spot at 47 kDa and isoelectric point (pI) of 4.9 is detected (figure 2).

1C1 does not cross-react with other enolases such as the a-enolase or the ß-enolase. 1C1 has been tested on MCF-7, MDA, COS and CCF. No staining is observed either on Western-Blot or immunofluorescence.

Instruction for use

For Western-Blotting, a final dilution of 1/5000 in TBS-Tween buffer is recommended.

For immunocytochemistry or immunofluorescence, a final dilution of 1/500 in PBS is recommended.

Note: For each application, the exact dilution should be determined. 1C1 has not been tested for immunoprecipitation.

FOR RESEARCH USE ONLY

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To obtain the antibody